为解决越来越多的强极性药物和小生物分子的分离难题,比较正规的足球外围app公司开发了一系列弱酸性、中性和碱性HILIC(Hydrophilic Interaction Chromatography)固定相来分离具有强极性的碱性、中性和酸性化合物。该类固定相是以高纯度具有良好机械稳定性的硅胶为基质,采用高纯度的键合试剂,可最大限度实现硅胶表面覆盖,从而确保了固定相的稳定性。通过运用独有的匀浆装填技术装填得到的HILIC柱柱床密度均一稳定,可保证具有最高的柱效。
比较正规的足球外围app公司的HILIC固定相类型包括Polar-100、Polar-Diol、Polar-Silica、Polar-Pyridine和Polar-Imidazole。Polar-100和Polar-Diol是中性的极性固定相,后者的极性要高于前者。Polar-Silica是弱酸性固定相。Polar-Pyridine和Polar-Imidazole是碱性固定相,后者的碱性要强于前者。
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | |
硅胶: | 球形, 高纯度(金属杂质<10 ppm) | ||||
---|---|---|---|---|---|
孔径: | 120 Å | ||||
粒径: | 1.8、2.2、3、4、5和10 µm | ||||
孔体积: | 1.0 mL/g | ||||
比表面积: | 300 m²/g | ||||
固定相结构: | 三官能团单分子层未封尾 | ||||
碳载量: | 0.0% | 16.0% | 8.8% | 8.0% | 8.6% |
键合密度: | 0 | 3.0 µmol/m² |
色谱柱: HILIC, 4.6 mm I.D.×150 mm, 5 µm
流动相: CH3CN: 10mM醋酸铵缓冲液=90:10 (v/v)
流速: 1.0 mL/min
检测波长: 254nm
进样量: 1 µL
温度: 室温
样品: 1.尿嘧啶, 2.腺苷(Adenosine), 3.尿苷(Uridine), 4.胞苷(Cytidine), 5.鸟苷(Guanosine)
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | ||
---|---|---|---|---|---|---|
长度×内径mm x mm | 粒径(µm) | 订货号 | ||||
30 × 2.1 | 3 | 130003-2103 | 131583-2103 | 133333-2103 | 134253-2103 | 135333-2103 |
50 × 2.1 | 3 | 130003-2105 | 131583-2105 | 133333-2105 | 134253-2105 | 135333-2105 |
100 × 4.6 | 3 | 130003-4610 | 131583-4610 | 133333-4610 | 134253-4610 | 135333-4610 |
150 × 4.6 | 3 | 130003-4615 | 131583-4615 | 133333-4615 | 134253-4615 | 135333-4615 |
250 × 4.6 | 3 | 130003-4625 | 131583-4625 | 133333-4625 | 134253-4625 | 135333-4625 |
30 × 2.1 | 5 | 130005-2103 | 131585-2103 | 133335-2103 | 134255-2103 | 135335-2103 |
50 × 2.1 | 5 | 130005-2105 | 131585-2105 | 133335-2105 | 134255-2105 | 135335-2105 |
100 × 4.6 | 5 | 130005-4610 | 131585-4610 | 133335-4610 | 134255-4610 | 135335-4610 |
150 × 4.6 | 5 | 130005-4615 | 131585-4615 | 133335-4615 | 134255-4615 | 135335-4615 |
250 × 4.6 | 5 | 130005-4625 | 131585-4625 | 133335-4625 | 134255-4625 | 135335-4625 |
150 × 10.0 | 5 | 130005-10015 | 131585-10015 | 133335-10015 | 134255-10015 | 135335-10015 |
250 × 10.0 | 5 | 130005-10025 | 131585-10025 | 133335-10025 | 134255-10025 | 135335-10025 |
150 × 21.2 | 5 | 130005-21215 | 131585-21215 | 133335-21215 | 134255-21215 | 135335-21215 |
250 × 21.2 | 5 | 130005-21225 | 131585-21225 | 133335-21225 | 134255-21225 | 135335-21225 |
150 × 30.0 | 5 | 130005-30015 | 131585-30015 | 133335-30015 | 134255-30015 | 135335-30015 |
250 × 30.0 | 5 | 130005-30025 | 131585-30025 | 133335-30025 | 134255-30025 | 135335-30025 |
150 × 10.0 | 10 | 130009-10015 | 131589-10015 | 133339-10015 | 134259-10015 | 135339-10015 |
250 × 10.0 | 10 | 130009-10025 | 131589-10025 | 133339-10025 | 134259-10025 | 135339-10025 |
150 × 21.2 | 10 | 130009-21215 | 131589-21215 | 133339-21215 | 134259-21215 | 135339-21215 |
250 × 21.2 | 10 | 130009-21225 | 131589-21225 | 133339-21225 | 134259-21225 | 135339-21225 |
150 × 30.0 | 10 | 130009-30005 | 131589-30015 | 133339-30015 | 134259-30015 | 135339-30015 |
250 × 30.0 | 10 | 130009-30010 | 131589-30025 | 133339-30025 | 134259-30025 | 135339-30025 |
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | |||
---|---|---|---|---|---|---|---|
长度×内径mm x mm | 粒径(µm) | 包装 | 订货号 | ||||
10 × 2.0 | 3 | 1/pk | 130003-2001 | 131583-2001 | 133333-2001 | 134253-2001 | 135333-2001 |
10 × 4.0 | 3 | 1/pk | 130003-4001 | 131583-4001 | 133333-4001 | 134253-4001 | 135333-4001 |
10 × 2.0 | 5 | 1/pk | 130005-2001 | 131585-2001 | 133335-2001 | 134255-2001 | 135335-2001 |
10 × 4.0 | 5 | 1/pk | 130005-4001 | 131585-4001 | 133335-4001 | 134255-4001 | 135335-4001 |
10 × 2.0 | 3 | 2/pk | 130003-2001T | 131583-2001T | 133333-2001T | 134253-2001T | 135333-2001T |
10 × 4.0 | 3 | 2/pk | 130003-4001T | 131583-4001T | 133333-4001T | 134253-4001T | 135333-4001T |
10 × 2.0 | 5 | 2/pk | 130005-2001T | 131585-2001T | 133335-2001T | 134255-2001T | 135335-2001T |
10 × 4.0 | 5 | 2/pk | 130005-4001T | 131585-4001T | 133335-4001T | 134255-4001T | 135335-4001T |
10 × 2.0 | 3 | 5/pk | 130003-2001F | 131583-2001F | 133333-2001F | 134253-2001F | 135333-2001F |
10 × 4.0 | 3 | 5/pk | 130003-4001F | 131583-4001F | 133333-4001F | 134253-4001F | 135333-4001F |
10 × 2.0 | 5 | 5/pk | 130005-2001F | 131585-2001F | 133335-2001F | 134255-2001F | 135335-2001F |
10 × 4.0 | 5 | 5/pk | 130005-4001F | 131585-4001F | 133335-4001F | 134255-4001F | 135335-4001F |
10 × 21.2 | 5 | 1/pk | 130005-21201 | 131585-21201 | 133335-21201 | 134255-21201 | 135335-21201 |
10 × 21.2 | 10 | 1/pk | 130009-21201 | 131589-21201 | 133339-21201 | 134259-21201 | 135339-21201 |
产品名称 | 包装(规格) | 订货号 |
---|---|---|
分析型保护柱套 | 10mm x2.0(4.0)mm | 102000-AH |
PEEK二接 | 7cm | 102002-P355 |
扳手 | 2个 | 102000-WRENCH |
制备型保护柱套 | 10mm x21.2mm | 102000-21201 |