Column:

GP-C18 ( 5 µm, 4.6 x 250 mm )

Mobile phase:

A: 0.35% H₃PO₄( adjust pH to 3.9 by NH₄OH ):90% ACN-CH₃OH ( 92 : 8 ) ,
B: 0.35% H₃PO₄( adjust pH to 3.9 by NH₄OH ):90% ACN-CH₃OH ( 52 : 48 )

Flow rate:

1.2 mL/min

Temperature:

40 ℃

Detection:

289 nm

Injection volume:

50 µL

Sample:

Clindamycin phosphate impurity reference in diluter
1. Lincomycin （林可霉素）6 μg/mL
2. Clindamycin phosphate （克林霉素磷酸酯）112.5 μg/mL
3. Clindamycin （克林霉素）30 μg/mL