Column:

Amethyst C18-H, 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:


Time(min):
B%:

A: 0.25% (CH₃)₄NOH
B: 0.25% (CH₃)₄NOH : CH₃CN : CH₃OH = 50 : 30 : 20 ( v/v )
0   2  22  32  37  38  48
5   5  25  50  50   5   5

Flow rate:

1.0 mL/min

Temperature:

40 ℃

Detection:

UV 254 nm

Injection volume:

20 µL

Sample:

0.2 mg/mL in mobile phase A
1. Cefdinir
2. E-isomer