应用编号(Application ID):PR0052
样品分类(Sample Category):医药(Pharmaceutical)
分离模式(Separation Mode):反相(Reversed phase chromatography)
产品系列(Product Line):BR
方法(Method):2010版中国药典方法
Column: |
BR-C18, 5 µm, 120 Å, 4.6 × 250 mm |
Mobile phase: |
A: 12% HAc : 0.2 M KH2PO4 : ACN : H2O = 0.5 : 50 : 50 : 900 (v/v); B: 12% HAc: 0.2 M KH2PO4 : ACN : H2O = 0.5 : 50 : 400 : 550 (v/v) |
Flow rate: |
1.0 mL/min |
Temperature: |
Ambient |
Detection: |
UV 254 nm |
Injection volume: |
20 µL |
Sample: |
Ampicillin (氨苄西林) |