应用编号(Application ID):
样品分类(Sample Category):医药(Pharmaceutical)
分离模式(Separation Mode):反相(Reversed phase chromatography)
产品系列(Product Line):GP
方法(Method):
Column: |
GP-C8 ( 5 µm, 120 Ǻ, 4.6 x 250 mm ) |
Mobile phase: |
phosphoric acid buffer ( 34 g phosphoric acid in 900 mL water, adjust to pH 6.1 with NH3·H2O and dilute to 1000 mL with water ) : methanol : acetonitrile = 75 : 8 : 17 (v/v) |
Flow rate: |
1 mL/min |
Temperature: |
50℃ |
Detection: |
UV 210 nm |
Injection volume: |
20µL |