应用编号（Application ID）：
样品分类（Sample Category）：医药（Pharmaceutical）
分离模式（Separation Mode）：反相（Reversed phase chromatography）
产品系列（Product Line）：GP
方法（Method）：
|
Column: |
GP C18 ( 5 µm, 120 Å, 4.6x 250 mm ) |
|
Mobile phase: |
dissolve 2.0 g of tetradecylammonium bromide R and 2.0 g of tetraheptylammonium bromide R in a mixture of 440 mL of water R, 55 mL of 0.067 M phosphate buffer solution pH 7.0 R, 5.0 mL of citrate buffer solution pH 5.0 prepared by dissolving 20.17 g of citric acid R in 800 mL of water R, adjusting to pH 5.0 with strong sodium hydroxide solution R and diluting to 1000.0 mL with water R, and 500 mL of acetonitrile R |
|
Flow rate: |
1.5 mL/min |
|
Column temperature: |
25 ℃ |
|
Detection: |
UV 254 nm |
|
Injection volume: |
20 µL |
