应用编号（Application ID）：
样品分类（Sample Category）：医药（Pharmaceutical）
分离模式（Separation Mode）：反相（Reversed phase chromatography）
产品系列（Product Line）：GP
方法（Method）：
|
Column: |
GP C18 ( 5 µm, 120 Å, 4.6 x 250 mm ) |
|
Mobile phase: |
Phosphate buffer pH 5.0 ( KH2PO4 2.0g dissolved by about 650mL water, add 15mL 0.5mol/L tetrabutylammonium hydroxide in methanol, 7mL1mol/L H3PO4 solution and 270mL methanol, cool to room temperature, adjust to pH 5.0 by NH3·H2O, then dilute to 1000mL by water ) |
|
Flow rate: |
1.2 mL/min |
|
Column temperature: |
25 ℃ |
|
Detection: |
UV 280 nm |
|
Injection volume: |
10 µL |
| Sample: | Folic Acid sample |
