Column:

HP-C18, 5 µm, 120 Å, 4.6 x 150 mm

Mobile phase:

A: Phosphate buffer ( pH 7.1 ) : Acetontrile = 98 : 2 ( v/v )

B: Phosphate buffer ( pH 7.1 ) : Acetontrile = 78 : 22 ( v/v )

      Time(min):
                   B%:

0      15       23       24         25
0     100     100       0          0

Flow rate:

1.5 mL/min

Temperature:

35 ℃

Detection:

UV 230 nm

Injection volume:

10 µL

Sample:

1 mg/mL Ranitidine Hydrochloride damaged by 50% Sodium hydroxide solution