Column:

Amethyst C18-H, 5 µm, 120 Å , 4.6 x 250 mm

Mobile phase:

Time( min ):
A%:

A: CH₃OH; B: 0.1% KH₂PO₄ : CH₃OH : TEA ( 35 : 65 : 0.2, v/v)
0   10   20   25   25.1   35
0   0     55   55   0         0

Flow rate:

0.8 mL/min

Temperature:

25 ^oC

Detection:

UV 230 nm

Injection volume:

20 µL

Sample:

0.5 mg/mL telmisartan （替米沙坦 ） in methanol