Column:

Amethyst C18-H, 5 µm, 120 Å, 4.6 × 250 mm

Mobile phase:

A: 4.0 mg/mL KH₂PO₄ ( adjust pH to 3.2 by H₃PO₄ )
B: A : ACN = 20 : 80 ( v/v )

      Time(min): 
                   B%:

0     15    25   30    45
45   75   80   100  100

Flow rate:

1.0 mL/min

Temperature:

45 ^oC

Detection:

UV 240 nm

Injection volume:

20 µL

Sample:

Repaglinide （瑞格列奈）